Module 2.
Entrez nucleotide and introduction to BLAST. Structure of GenBank, tour of the various sections nr, ESTdb, GSS, HTGS. UNIGENE and COGS, two additional resources at GenBank. Use of the taxonomy browser, how to find out how many sequences are present for a given organism, how many are EST sequences, how many are genomic, how many are mRNA. Retrieving DNA and protein sequences via the web. Introduction to the basic BLAST algorithm, how it works, how to use it, how to interpret your results.
Objectives: Introduce the four main sections of the Entrez nucleotide interface to GenBank. Understand what is there and how to find it. Explain what a BLAST search is and demonstrate the power of this tool. Understand BLAST output and the significance of the scores and alignments.
Overview (revised)
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